What does a positive immunofixation mean?

What does a positive immunofixation mean?

Positive results from the test indicate the presence of abnormal Ig. This may suggest the existence of an underlying health condition, such as: an immune system disorder. multiple myeloma. Waldenstrom’s macroglobulinemia.

What is an immunofixation urine test?

Urine immunofixation is a test that measures proteins in your urine. It specifically looks for monoclonal protein, or M-protein, which shouldn’t be there in most cases.‌ Doctors will be able to find out if you have conditions such as multiple myeloma or primary amyloidosis through urine immunofixation.

When should I buy Immunofixation?

Immunofixation is clearly indicated upon clinical or laboratory evidence of a plasma cell dyscrasia for the diagnosis of multiple myeloma, Waldenstrom macroglobulinemia, or amyloid light-chain (AL) amyloidosis. Typically, the monoclonal protein is discovered, and workup then ensues.

How is Immunofixation performed?

The technique consists of depositing a serum (or urine which has been previously concentrated) sample on a gel. After application of an electric current that allows the separation of proteins according to their size, antibodies specific for each type of immunoglobulin are laid upon the gel.

When should I buy immunofixation?

What do you need to know about immunofixation blood test?

What is an immunofixation (IFE) blood test? An immunofixation blood test, also known as protein electrophoresis, measures certain proteins in the blood. Proteins play many important roles, including providing energy for the body, rebuilding muscles, and supporting the immune system.

What happens during the fixation phase of immunofixation?

Immunofixation consists of an electrophoresis phase and a fixation phase. Serum or urine immunofixation negative for a monoclonal protein or a polyclonal pattern is considered to be normal.

What is the purpose of immunofixation in gammopathy?

Immunofixation should be used to confirm the presence of an M protein and to identify the heavy-chain type and the light-chain class.1 In immunofixation, electrophoresis of one specimen from a patient suspected of monoclonal gammopathy is performed using five separate lanes.

How is immunofixation used to determine the isotype of a protein?

In addition, the immunofixation technique can also determine the particular isotype of the monoclonal protein. However, the immunofixation technique cannot be used to estimate the quantity of the M protein. In contrast, serum protein electrophoresis is capable of estimating the concentration of an M protein.

Why is it important to know about immunofixation?

From Wikipedia, the free encyclopedia Immunofixation permits the detection and typing of monoclonal antibodies or immunoglobulins in serum or urine. It is of great importance for the diagnosis and monitoring of certain blood related diseases such as myeloma.

When is an immunofixation test indicated?

In addition, when protein electrophoresis assays are positive for a monoclonal protein, serum and urine immunofixation are indicated for the appropriate identification of the immunoglobulin and the corresponding light chain.

How does immunofixation work on a computer screen?

Immunofixation “fixes” certain proteins into place with antibodies and then washes away the others before staining them. On a computer screen, these proteins form a pattern of bands. Peaks and valleys in the bands may mean that you may be making too many or too few of certain proteins.

When do you need an Immunofixation electrophoresis test?

Immunofixation electrophoresis is usually ordered when the protein electrophoresis test shows the presence of an abnormal protein band that may be an immunoglobulin. Electrophoresis tests are most frequently requested when a doctor suspects a disease or condition that causes a monoclonal protein to be produced.